human cd36+ cells  (AllCells LLC)

Journal: PLOS ONE

Article Title: Monocyte-cancer cell fusion is mediated by phosphatidylserine—CD36 receptor interaction and induced by ionizing radiation

doi: 10.1371/journal.pone.0311027

Figure Lengend Snippet: Fluorescent microscopy of CellTrace and CD36 staining A) MCF-7 cells stained with CellTrace Far red B) THP-1 cells stained with CellTrace Violet C) THP-1 cells stained with CD36 antibodies. All images were taken with x40 magnification.

Article Snippet: The cell cultures were washed in PBS, incubated with Human TruStain FcX TM (Fc Receptor Blocking Solution) (BioLegend, 422302, San Diego, USA) for 10 minutes at 4°C, 1:20 ratio to Cell Staining Buffer (Nordic Biosite, ASB-022501L, Täby, Sweden), followed by staining with 5 μg/ml Anti-Human CD36/SCARB3 Antibody (Phycoerythrin (PE)-conjugated) (Rabbit monoclonal IgG antibody, Sino Biological, 10752-R001-P, Beijing, China), or 50 μg/ml Rabbit IgG Isotype Control, PE-conjugated (Bioss, bs-0295P-PE, Massachusetts, USA) for 30 minutes in darkness at 4°C.

Techniques: Microscopy, Staining

Journal: PLOS ONE

Article Title: Monocyte-cancer cell fusion is mediated by phosphatidylserine—CD36 receptor interaction and induced by ionizing radiation

doi: 10.1371/journal.pone.0311027

Figure Lengend Snippet: Panel A shows THP-1 and MCF-7 cells cultured in separate flasks and treated with 0, 2.5, 5, and 10 Gy radiation. After that, the cells were analyzed by flow cytometry to detect the proportion of cells expressing PS and CD36. Panel B shows a cell fusion experiment where THP-1 cells (labelled with CellTrace Violet) and MCF-7 cells (labelled with CellTracer Far Red) are grown in the same flask and treated with 0 and 5 Gy gamma radiation. After 2 days of co-culture, the cells are sorted with FACS Aria III. Cells co-expressing CellTrace Violet and Far Red are assessed as THP1-/MCF-5 tumor hybrid cells.

Article Snippet: The cell cultures were washed in PBS, incubated with Human TruStain FcX TM (Fc Receptor Blocking Solution) (BioLegend, 422302, San Diego, USA) for 10 minutes at 4°C, 1:20 ratio to Cell Staining Buffer (Nordic Biosite, ASB-022501L, Täby, Sweden), followed by staining with 5 μg/ml Anti-Human CD36/SCARB3 Antibody (Phycoerythrin (PE)-conjugated) (Rabbit monoclonal IgG antibody, Sino Biological, 10752-R001-P, Beijing, China), or 50 μg/ml Rabbit IgG Isotype Control, PE-conjugated (Bioss, bs-0295P-PE, Massachusetts, USA) for 30 minutes in darkness at 4°C.

Techniques: Cell Culture, Flow Cytometry, Expressing, Co-Culture Assay

Journal: PLOS ONE

Article Title: Monocyte-cancer cell fusion is mediated by phosphatidylserine—CD36 receptor interaction and induced by ionizing radiation

doi: 10.1371/journal.pone.0311027

Figure Lengend Snippet: The proportion of MCF-7 expressing PS increases in dose-dependent relation (C). No correlation of PS and CD36 expression in THP-1 cells nor CD36 expression in MCF-7 cells was found in relation to the dose of ionizing radiation.

Article Snippet: The cell cultures were washed in PBS, incubated with Human TruStain FcX TM (Fc Receptor Blocking Solution) (BioLegend, 422302, San Diego, USA) for 10 minutes at 4°C, 1:20 ratio to Cell Staining Buffer (Nordic Biosite, ASB-022501L, Täby, Sweden), followed by staining with 5 μg/ml Anti-Human CD36/SCARB3 Antibody (Phycoerythrin (PE)-conjugated) (Rabbit monoclonal IgG antibody, Sino Biological, 10752-R001-P, Beijing, China), or 50 μg/ml Rabbit IgG Isotype Control, PE-conjugated (Bioss, bs-0295P-PE, Massachusetts, USA) for 30 minutes in darkness at 4°C.

Techniques: Expressing

Journal: PLOS ONE

Article Title: Monocyte-cancer cell fusion is mediated by phosphatidylserine—CD36 receptor interaction and induced by ionizing radiation

doi: 10.1371/journal.pone.0311027

Figure Lengend Snippet: The proportion of THP-1/MCF-7 tumor hybrid cells in co-cultures supplemented with 40 μg/ml and 80 μg/ml CD36 antibody was 1.8% and 1.9%, respectively. In non-treated THP-1/MCF-7 co-cultures, the proportion of tumor hybrid cells (3.6%) was significantly higher compared to co-cultures treated with anti-CD36 antibody (P<0.001).

Article Snippet: The cell cultures were washed in PBS, incubated with Human TruStain FcX TM (Fc Receptor Blocking Solution) (BioLegend, 422302, San Diego, USA) for 10 minutes at 4°C, 1:20 ratio to Cell Staining Buffer (Nordic Biosite, ASB-022501L, Täby, Sweden), followed by staining with 5 μg/ml Anti-Human CD36/SCARB3 Antibody (Phycoerythrin (PE)-conjugated) (Rabbit monoclonal IgG antibody, Sino Biological, 10752-R001-P, Beijing, China), or 50 μg/ml Rabbit IgG Isotype Control, PE-conjugated (Bioss, bs-0295P-PE, Massachusetts, USA) for 30 minutes in darkness at 4°C.

Techniques:

Journal: Biophysical Journal

Article Title: Temperature-Induced Catch-Slip to Slip Bond Transit in Plasmodium falciparum -Infected Erythrocytes

doi: 10.1016/j.bpj.2019.11.016

Figure Lengend Snippet: Results from single bond force-clamp spectroscopy experiments conducted at 25, 37, and 41°C. Single bond lifetime τ against force of IRBC-ICAM-1 and IRBC-CD36 bonds at 25°C (a and b) (13), 37°C (c and d), and 41°C (e and f) fitted to their corresponding catch-slip and slip bond models (solid lines). Single bond lifetimes are plotted as mean ± SEM. Insets are plots of the survival probability P of IRBC-ICAM-1 and IRBC-CD36 interactions at 37°C (c and d) and 41°C (e and f) fitted to straight lines (solid lines) via least-square method (bi-square weights) to indicate first order kinetics. To see this figure in color, go online.

Article Snippet: Thereafter, the tip was incubated for 1 h with either 10 μ L of human recombinant ICAM-1 protein—5 μ g/mL for force-clamp spectroscopy at 37°C and 2.5 μ g/mL for 41°C—or CD36 (Sino Biological)—2 μ g/mL for both temperatures.

Techniques: Spectroscopy

Journal: Biophysical Journal

Article Title: Temperature-Induced Catch-Slip to Slip Bond Transit in Plasmodium falciparum -Infected Erythrocytes

doi: 10.1016/j.bpj.2019.11.016

Figure Lengend Snippet: Fitting Parameters for Single Bond Force-Clamp Spectroscopy and Flow Assay Obtained at Body and Febrile Temperatures

Article Snippet: Thereafter, the tip was incubated for 1 h with either 10 μ L of human recombinant ICAM-1 protein—5 μ g/mL for force-clamp spectroscopy at 37°C and 2.5 μ g/mL for 41°C—or CD36 (Sino Biological)—2 μ g/mL for both temperatures.

Techniques: Spectroscopy, Binding Assay

Journal: Biophysical Journal

Article Title: Temperature-Induced Catch-Slip to Slip Bond Transit in Plasmodium falciparum -Infected Erythrocytes

doi: 10.1016/j.bpj.2019.11.016

Figure Lengend Snippet: Results from multiple bond flow assay obtained at 37 and 41°C. Shown is the cellular lifetime against shear stress for IRBC-ICAM-1 and IRBC-CD36 bonds at 37°C (a and b) and 41°C (c and d). Shown is an overlay of cellular lifetime against force graphs of IRBC-ICAM-1 and IRBC-CD36 interactions at all three temperatures (e and f). Blue, green, and red are used to distinguish the experimental data and fitting obtained at 25°C (13), 37°C, and 41°C, respectively. Cellular lifetimes are plotted as mean ± SEM. To see this figure in color, go online.

Article Snippet: Thereafter, the tip was incubated for 1 h with either 10 μ L of human recombinant ICAM-1 protein—5 μ g/mL for force-clamp spectroscopy at 37°C and 2.5 μ g/mL for 41°C—or CD36 (Sino Biological)—2 μ g/mL for both temperatures.

Techniques:

Journal: PLoS ONE

Article Title: The Use of Wavelength Modulated Raman Spectroscopy in Label-Free Identification of T Lymphocyte Subsets, Natural Killer Cells and Dendritic Cells

doi: 10.1371/journal.pone.0125158

Figure Lengend Snippet: (a) CD4 staining of isolated CD4 + T cells. (b) IL-2 ELISA of CD4 + T cells stimulated with or without anti-CD3/CD28 beads. (c) CD8 staining or isolated CD8 + T cells. (d) IFNγ ELISPOT assay of PBMC and purified CD8 + T cells incubated with and without EBV derived peptide AVFDRKSDAK. (e) CD56 staining of isolated NK cells. (f) NK cell degranulation assay—CD107a staining of NK cells incubated without (left panel) or with (right panel) MHC class I deficient. 221 cells at a 10:1 effector to target ratio. (g) CD303 staining of isolated pDC. (h) CD1c staining of isolated mDC. The x-axis in each flow cytometry plot indicates fluorescent intensity. The left hand peak in each flow cytometry plot indicates control staining with an irrelevant antibody. Representative white-light microscopy images of each of the purified cell populations used in Raman spectroscopy experiments are also shown.

Article Snippet: Cells were isolated using Dynabeads (Life Technologies) untouched human CD4 T cell kit (depleting antibodies comprising anti-CD8, CD14, CD16a, CD16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human CD8 T cell kit (depleting antibodies comprising anti-CD4, CD14, CD16a, Cd16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human NK cell kit (depleting antibodies comprising anti-CD3, CD14, CD36, HLA Class II, CD123 and CD235a).

Techniques: Staining, Isolation, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot, Purification, Incubation, Derivative Assay, Degranulation Assay, Flow Cytometry, Light Microscopy, Raman Spectroscopy

Journal: PLoS ONE

Article Title: The Use of Wavelength Modulated Raman Spectroscopy in Label-Free Identification of T Lymphocyte Subsets, Natural Killer Cells and Dendritic Cells

doi: 10.1371/journal.pone.0125158

Figure Lengend Snippet: (a) Mean standard Raman spectra of CD4 + , CD8 + and CD56 + NK cells. (b)-(d) Pairwise comparison of the WMRS spectra obtained from purified lymphocyte subsets. (b) Mean spectra of CD4 + versus CD8 + T cells. (c) Mean spectra of CD4 + T cells versus CD56 + NK cells. (d) Mean spectra of CD8 + T cells versus CD56 + NK cells. Solid spectra lines represent mean of each subset, with shadow lines representing the standard deviation. Shaded vertical bands indicated regions of significant difference, estimated by student’s T test at level of p<10 –7 .

Article Snippet: Cells were isolated using Dynabeads (Life Technologies) untouched human CD4 T cell kit (depleting antibodies comprising anti-CD8, CD14, CD16a, CD16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human CD8 T cell kit (depleting antibodies comprising anti-CD4, CD14, CD16a, Cd16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human NK cell kit (depleting antibodies comprising anti-CD3, CD14, CD36, HLA Class II, CD123 and CD235a).

Techniques: Purification, Standard Deviation

Journal: PLoS ONE

Article Title: The Use of Wavelength Modulated Raman Spectroscopy in Label-Free Identification of T Lymphocyte Subsets, Natural Killer Cells and Dendritic Cells

doi: 10.1371/journal.pone.0125158

Figure Lengend Snippet: (a) CD4 + , CD8 + T cells and CD56 + NK cells. (b) CD4 + and CD8 + T cells. (c) CD4 + T cells and CD56 + NK cells. (d) CD8 + T cells and CD56 + NK cells. (3D rotating views of these plots are available to view in the supplementary information).

Article Snippet: Cells were isolated using Dynabeads (Life Technologies) untouched human CD4 T cell kit (depleting antibodies comprising anti-CD8, CD14, CD16a, CD16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human CD8 T cell kit (depleting antibodies comprising anti-CD4, CD14, CD16a, Cd16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human NK cell kit (depleting antibodies comprising anti-CD3, CD14, CD36, HLA Class II, CD123 and CD235a).

Techniques:

Journal: PLoS ONE

Article Title: The Use of Wavelength Modulated Raman Spectroscopy in Label-Free Identification of T Lymphocyte Subsets, Natural Killer Cells and Dendritic Cells

doi: 10.1371/journal.pone.0125158

Figure Lengend Snippet: (a) CD4 + T cells. (b) CD8 + T cells. (c) CD56 + NK cells. (3D rotating views of these plots are available to view in the supplementary information).

Article Snippet: Cells were isolated using Dynabeads (Life Technologies) untouched human CD4 T cell kit (depleting antibodies comprising anti-CD8, CD14, CD16a, CD16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human CD8 T cell kit (depleting antibodies comprising anti-CD4, CD14, CD16a, Cd16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human NK cell kit (depleting antibodies comprising anti-CD3, CD14, CD36, HLA Class II, CD123 and CD235a).

Techniques:

Journal: PLoS ONE

Article Title: The Use of Wavelength Modulated Raman Spectroscopy in Label-Free Identification of T Lymphocyte Subsets, Natural Killer Cells and Dendritic Cells

doi: 10.1371/journal.pone.0125158

Figure Lengend Snippet: (a) CD4 staining of isolated CD4 + T cells. (b) IL-2 ELISA of CD4 + T cells stimulated with or without anti-CD3/CD28 beads. (c) CD8 staining or isolated CD8 + T cells. (d) IFNγ ELISPOT assay of PBMC and purified CD8 + T cells incubated with and without EBV derived peptide AVFDRKSDAK. (e) CD56 staining of isolated NK cells. (f) NK cell degranulation assay—CD107a staining of NK cells incubated without (left panel) or with (right panel) MHC class I deficient. 221 cells at a 10:1 effector to target ratio. (g) CD303 staining of isolated pDC. (h) CD1c staining of isolated mDC. The x-axis in each flow cytometry plot indicates fluorescent intensity. The left hand peak in each flow cytometry plot indicates control staining with an irrelevant antibody. Representative white-light microscopy images of each of the purified cell populations used in Raman spectroscopy experiments are also shown.

Article Snippet: Cells were isolated using Dynabeads (Life Technologies) untouched human CD4 T cell kit (depleting antibodies comprising anti-CD8, CD14, CD16a, CD16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human CD8 T cell kit (depleting antibodies comprising anti-CD4, CD14, CD16a, Cd16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human NK cell kit (depleting antibodies comprising anti-CD3, CD14, CD36, HLA Class II, CD123 and CD235a).

Techniques: Staining, Isolation, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot, Purification, Incubation, Derivative Assay, Degranulation Assay, Flow Cytometry, Light Microscopy, Raman Spectroscopy

Journal: PLoS ONE

Article Title: The Use of Wavelength Modulated Raman Spectroscopy in Label-Free Identification of T Lymphocyte Subsets, Natural Killer Cells and Dendritic Cells

doi: 10.1371/journal.pone.0125158

Figure Lengend Snippet: (a) Mean standard Raman spectra of CD4 + , CD8 + and CD56 + NK cells. (b)-(d) Pairwise comparison of the WMRS spectra obtained from purified lymphocyte subsets. (b) Mean spectra of CD4 + versus CD8 + T cells. (c) Mean spectra of CD4 + T cells versus CD56 + NK cells. (d) Mean spectra of CD8 + T cells versus CD56 + NK cells. Solid spectra lines represent mean of each subset, with shadow lines representing the standard deviation. Shaded vertical bands indicated regions of significant difference, estimated by student’s T test at level of p<10 –7 .

Article Snippet: Cells were isolated using Dynabeads (Life Technologies) untouched human CD4 T cell kit (depleting antibodies comprising anti-CD8, CD14, CD16a, CD16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human CD8 T cell kit (depleting antibodies comprising anti-CD4, CD14, CD16a, Cd16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human NK cell kit (depleting antibodies comprising anti-CD3, CD14, CD36, HLA Class II, CD123 and CD235a).

Techniques: Purification, Standard Deviation

Journal: PLoS ONE

Article Title: The Use of Wavelength Modulated Raman Spectroscopy in Label-Free Identification of T Lymphocyte Subsets, Natural Killer Cells and Dendritic Cells

doi: 10.1371/journal.pone.0125158

Figure Lengend Snippet: (a) CD4 + , CD8 + T cells and CD56 + NK cells. (b) CD4 + and CD8 + T cells. (c) CD4 + T cells and CD56 + NK cells. (d) CD8 + T cells and CD56 + NK cells. (3D rotating views of these plots are available to view in the supplementary information).

Article Snippet: Cells were isolated using Dynabeads (Life Technologies) untouched human CD4 T cell kit (depleting antibodies comprising anti-CD8, CD14, CD16a, CD16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human CD8 T cell kit (depleting antibodies comprising anti-CD4, CD14, CD16a, Cd16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human NK cell kit (depleting antibodies comprising anti-CD3, CD14, CD36, HLA Class II, CD123 and CD235a).

Techniques:

Journal: PLoS ONE

Article Title: The Use of Wavelength Modulated Raman Spectroscopy in Label-Free Identification of T Lymphocyte Subsets, Natural Killer Cells and Dendritic Cells

doi: 10.1371/journal.pone.0125158

Figure Lengend Snippet: Confusion matrix for CD4 + , CD8 + and CD56 + cell subsets.

Article Snippet: Cells were isolated using Dynabeads (Life Technologies) untouched human CD4 T cell kit (depleting antibodies comprising anti-CD8, CD14, CD16a, CD16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human CD8 T cell kit (depleting antibodies comprising anti-CD4, CD14, CD16a, Cd16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human NK cell kit (depleting antibodies comprising anti-CD3, CD14, CD36, HLA Class II, CD123 and CD235a).

Techniques:

Journal: PLoS ONE

Article Title: The Use of Wavelength Modulated Raman Spectroscopy in Label-Free Identification of T Lymphocyte Subsets, Natural Killer Cells and Dendritic Cells

doi: 10.1371/journal.pone.0125158

Figure Lengend Snippet: (a) CD4 + T cells. (b) CD8 + T cells. (c) CD56 + NK cells. (3D rotating views of these plots are available to view in the supplementary information).

Article Snippet: Cells were isolated using Dynabeads (Life Technologies) untouched human CD4 T cell kit (depleting antibodies comprising anti-CD8, CD14, CD16a, CD16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human CD8 T cell kit (depleting antibodies comprising anti-CD4, CD14, CD16a, Cd16b, CD19, CD36, CD56, CD123 and CD235a), Dynabeads untouched human NK cell kit (depleting antibodies comprising anti-CD3, CD14, CD36, HLA Class II, CD123 and CD235a).

Techniques: